G2536
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G2536
Sigma
Ganciclovir
≥99% (HPLC), powder
| CAS Number: | 82410-32-0 |
| Linear Formula: | C9H13N5O4 |
| Molecular Weight: | 255.23 |
| MDL number: | MFCD00870588 |
| PubChem Substance ID: | 24278453 |
Description
| Application | Ganciclovir is used in molecular biology for selection against random recombination events when homologous recombination of a gene of interest is required. |
| Biochem/physiol Actions | Ganciclovir is a pro-drug nucleoside analog that is activated by phosphorylation. It is useful in the study of gene therapy in cancer research. Upon expression of a viral suicide gene encoding thymidine kinase, the non-toxic pro-drug is converted to a phosphorylated active analog and is incorporated into the DNA of replicating eukaryotic cells, causing death of the malignant dividing cell.1 The cell cycle is irreversibly arrested at the G2-M checkpoint.2 Gap junction involvement in the ganciclovir bystander effect has been studied.3 Ganciclovir has been used to study loss of telomeres4 and to evaluate sensitivity of viruses to antiviral treatments.5 |
| Ganciclovir is a pro-drug nucleoside analog that is activated by phosphorylation. It is useful in the study of gene therapy in cancer research. |
Properties
| assay | ≥99% (HPLC) |
| form | powder |
| color | white |
| solubility | 0.1 M HCl: 10 mg/mL |
| abs. | ε |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type P2 (EN 143) respirator cartridges |
| Hazard Codes | T |
| Risk Statements | 46-60-61 |
| Safety Statements | 53-36/37/39-45 |
| WGK Germany | 3 |
| RTECS | MF8407000 |
References
| Cited Reference | 1. Rubsam, L.Z., et al., Cytotoxicity and accumulation of ganciclovir triphosphate in bystander cells cocultured with herpes simplex virus type 1 thymidine kinase-expressing human glioblastoma cells. Cancer Res. 59, 675, (1999) |
| 2. Halloran, P.J., and Fenton, R.G., Irreversible G2-M arrest and cytoskeletal reorganization induced by cytotoxic nucleoside analogues. Cancer Res. 58, 3855-3865, (1998) Abstract | |
| 3. Yamasaki, H., et al., Role of connexin (gap junction) genes in cell growth control and carcinogenesis. C.R. Acad. Sci. III 322, 151-159, (1999) Abstract | |
| 4. Sprung, C.N., et al., Chromosome healing in mouse embryonic stem cells. Proc. Natl. Acad. Sci. U. S. A. 96, 6781-6786, (1999) Abstract | |
| 5. Oon, C.J., et al., Hepatitis B virus variants with lamivudine-related mutations in the DNA polymerase and the 'a' epitope of the surface antigen are sensitive to ganciclovir. Antiviral Res. 41, 113-118, (1999) Abstract | |
| reference | Cannon, J.S., et al., Human herpes virus 8-encoded thymidine kinase and phosphotransferase homologues confer sensitivity to ganciclovir. J. Virol. 73, 4786-4793, (1999) Abstract |
